| Numéro | Pilote | Nationalité | Moto | Team | Portrait |
| 1 | Kenny Roberts | 
USA |
Suzuki | Telefonica Movistar Suzuki |  |
| 3 | Max Biaggi | 
ITA |
Yamaha | Marlboro Yamaha Team |  |
| 4 | Alex Barros | 
BRA |
Honda | West Honda Pons |  |
| 5 | Garry McCoy | 
AUS |
Yamaha | Red Bull Yamaha WCM |  |
| 6 | Norifumi Abe | 
JPN |
Yamaha | Antena 3 Yamaha - D'Antin |  |
| 7 | Carlos Checa | 
SPA |
Yamaha | Marlboro Yamaha Team |  |
| 8 | Chris Walker | 
GBR |
Honda | Shell Advance Racing |  |
| 9 | Leon Haslam |
GBR |
Honda | Shell Advance Racing |  |
| 10 | Jose-Luise Cardoso |
SPA |
Yamaha | Antena 3 Yamaha - D'Antin |  |
| 11 | Tohru Ukawa |
JPN |
Honda | Repsol - YPF Honda Team |  |
| 12 | Haruchika Aoki |
JPN |
Honda | Arie Molenaar Racing |  |
| 14 | Anthony West |
AUS |
Honda | Dee Cee Jeans Racing Team |  |
| 15 | Sete Gibernau |
SPA |
Suzuki | Telefonica Movistar Suzuki |  |
| 16 | Johan Stigefelt | 
SWE |
Sabre V4 | Sabre Sport |  |
| 17 | Jurgen V. D. Goorbergh | 
NED |
Proton KR3 | Proton Team KR |  |
| 19 | Olivier Jacque | 
FRA |
Yamaha | Gauloises Yamaha Tech 3 |  |
| 21 | Barry Veneman |
NED |
Honda | Dee Cee Jeans Racing Team |  |
| 24 | Jason Vincent |
GBR |
Pulse | Pulse Racing |  |
| 28 | Alex Criville |
SPA |
Honda | Repsol - YPF Honda Team |  |
| 41 | Noriyuki Haga |
JPN |
Yamaha | Red Bull Yamaha WCM |  |
| 46 | Valentino Rossi |
ITA |
Honda | Nastro Azzuro Honda |  |
| 56 | Shinya Nakano |
JPN |
Yamaha | Gauloises Yamaha Tech 3 |  |
| 65 | Loris Capirossi |
ITA |
Honda | West Honda Pons |  |
| 68 | Mark Willis |
AUS |
Pulse | Pulse Racing |  |
LES ENGAGES 2001 EN GP 500